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For example, genes that encode proteins capable of breaking down mycotoxins (Karlovsky, 2011) or inhibiting the activity of cell-wall-degrading enzymes can be introduced into plants. Plants can be engineered to synthesize and secrete antimicrobial peptides or compounds that directly inhibit colonization ( Osusky et al., 2000 ).
- Genome-Editing Technologies: Principles and Applications
Targeted nucleases have provided researchers with the...
- Genome-Editing Technologies: Principles and Applications
15 Δεκ 2022 · The efficient delivery of gene-modification components into plant cells is a crucial first step of plant transformation and genome editing. Two major gene-delivery methodologies have...
24 Απρ 2017 · This proof-of-concept study demonstrated that it is possible to replace a wild-type gene with an altered one using CRISPR-Cas9 in plant cells. A year later, germline editing of the ADH1 gene was demonstrated in Arabidopsis [ 86 ].
25 Μαΐ 2020 · The results indicates that multiplex genome editing using CRISPR/Cas9 can be used to mimic the domestication process during evolution in a short time frame, with implications for a rapid and convenient generation of new plants with desirable traits.
28 Νοε 2019 · Major concerns include the spread of recombinant genes through seed dispersal, pollen dispersal, viral transfer or horizontal transfer; therapeutic proteins getting into the food supply of humans or animals; and adverse effects on organisms in the environment (Ma et al., 2003; Obembe et al., 2011).
Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy.
16 Ιουν 2023 · CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops.
