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  1. This chapter provides a brief description of genetic modification methods used to develop new plant, animal, and microbial strains for use as human food. The next chapter (Chapter 3) presents a detailed analysis of the likelihood for these methods to result in unintentional compositional changes.

  2. Plant-derived antimicrobial peptides and other compounds can suppress pathogenicity by direct detoxification or through inhibition of the activity of virulence factors (Kitajima and Sato, 1999; Thomma et al., 2002; Ahuja et al., 2012).

  3. 10 Μαρ 2023 · Main approaches used to create stable transgenic plants include (i) biolistic bombardment of plant cells and tissues with DNA coated nanoparticles using a gene gun, and (ii) Agrobacterium mediated transformation.

  4. Abstract. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide range of species, including plants.

  5. 28 Νοε 2019 · Major concerns include the spread of recombinant genes through seed dispersal, pollen dispersal, viral transfer or horizontal transfer; therapeutic proteins getting into the food supply of humans or animals; and adverse effects on organisms in the environment (Ma et al., 2003; Obembe et al., 2011).

  6. When delivered into plant cells, SSN locate and cleave the target DNA, resulting in double-strand breaks (DSBs) that are repaired by natural DNA repair mechanisms like non-homologous end-joining (NHEJ) and homology-directed repair (HDR).

  7. CRISPR-Cas9 could be used to modify targeted genes in herbal plants, survey the synthesis of efficacious com-pounds, select characteristics for increased yield, and advance research on biosynthetic pathways and regulatory mechanisms now that the genomes of some medicinal plants have been fully sequenced .

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