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For example, genes that encode proteins capable of breaking down mycotoxins (Karlovsky, 2011) or inhibiting the activity of cell-wall-degrading enzymes can be introduced into plants. Plants can be engineered to synthesize and secrete antimicrobial peptides or compounds that directly inhibit colonization ( Osusky et al., 2000 ).
- Technological Development and Application of Plant Genetic ...
The main bottleneck in successful plant genetic...
- Technological Development and Application of Plant Genetic ...
9 Φεβ 2022 · Precise, efficient, and rapid genome editing technique is revolutionizing crop improvement by avoiding the genetic modification, gene disruption, and introduction unwanted genes (such as selectable marker genes).
3 Ιουλ 2017 · During the past few years, several novel genome editing systems have been developed; these include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9).
25 Μαΐ 2020 · The results indicates that multiplex genome editing using CRISPR/Cas9 can be used to mimic the domestication process during evolution in a short time frame, with implications for a rapid and convenient generation of new plants with desirable traits.
10 Μαρ 2023 · Main approaches used to create stable transgenic plants include (i) biolistic bombardment of plant cells and tissues with DNA coated nanoparticles using a gene gun, and (ii) Agrobacterium mediated transformation.
The main bottleneck in successful plant genetic transformation is how biomolecules enter plant cells through the hard multi-layer cell wall and the subsequent regeneration of transgenic plants from an in vitro cultured explant, either via de novo organogenesis or somatic embryogenesis .
Abstract. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide range of species, including plants.
