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For example, genes that encode proteins capable of breaking down mycotoxins (Karlovsky, 2011) or inhibiting the activity of cell-wall-degrading enzymes can be introduced into plants. Plants can be engineered to synthesize and secrete antimicrobial peptides or compounds that directly inhibit colonization ( Osusky et al., 2000 ).
Abstract. The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide range of species, including plants.
15 Αυγ 2020 · Gene therapy involves changing the genetic information in a cell. Stem cells are cells that can divide and differentiate into the desired cell type. It is possible to do gene therapy on stem cells. One approach used in the work on treating muscular dystrophy in dogs was of this type.
1 Μαΐ 2019 · Multiplex gene editing systems in plants. (A) Vector systems developed for multiplex gene editing in plants. Pol II/III pro: RNA Pol II-or III-dependent gene promoters; gRNA: guide RNA;...
24 Απρ 2017 · This proof-of-concept study demonstrated that it is possible to replace a wild-type gene with an altered one using CRISPR-Cas9 in plant cells. A year later, germline editing of the ADH1 gene was demonstrated in Arabidopsis [ 86 ].
The DNA binding domain of TALENs corresponds to a single TALE protein and a single base pair. In 2014, the recognition preferences of 400 unique RVDs were decoded, making TALENs a valuable tool for researchers studying gene function and gene therapy (Yang et al. 2014).
3 Ιουλ 2017 · The simplified approach to gene/genome editing represents a valuable tool for plant researchers in functional analysis of gene(s) and for breeders in the integration of key genes in the genomes of agriculturally important crops.
