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Spectroscopy requires the same basic calibration data as for photometry, but with a few important additions (for example, wavelength calibration spectra). Also, some of the calibration steps, while similar in purpose to the analogous ones for photometry, must be handled different (for example, flat fielding).
11 Αυγ 2022 · Absorbance Spectroscopy. A spectrophotometer in an instrument that measures the amount of light absorbed at a specific wavelength (λ) by a sample, and can be used to generate a spectrum, which is a plot of the absorbance as a function of the wavelength.
The wavelength, λ, is defined as the distance between successive maxima (see Figure 10.1.2 10.1. 2). For ultraviolet and visible electromagnetic radiation the wavelength is usually expressed in nanometers (1 nm = 10 –9 m), and for infrared radiation it is given in microns (1 μm = 10 –6 m).
10 Αυγ 2022 · A wavelength selector is a instrument component that either selects and transmits a narrow band of wavelengths emanating from a broad band optical source of transmits one or more lines from a discrete wavelength source.
The key components of a spectrophotometer are: A light source that generates a broadband of electromagnetic radiation across the UV-visible spectrum. A dispersion device separates the broadband radiation into wavelengths. A sample area, where the light passes through or reflects off a sample.
There are a great many ways in which spectroscopy may be conducted. In some cases, light of different wavelengths is shined on a sample and the wavelengths that get absorbed most strongly are measured. In others, you let the sample first absorb light and then measure the wavelength of light emitted.
Edmund Optics offers both single surface and dual surface optical flats in either ZERODUR® or Fused Silica. The flatness of an optical flat is measured in fractions of a reference wavelength, 632.8nm. A λ/20 flat will have a maximum peak to valley deviation of 632.8/20 or 31.64nm.
