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A flow cytometer is composed of fluidic, optic, and electronic systems. This chapter explains the role of each system, and how they work together. You will also learn the basics of electrostatic cell sorting.
Flow Cytometry » Flow Cytometry is the technical process that allows for the individual measurements of cell fluorescence and light scattering. This process is performed at rates of thousands of cells per second. » This information can be used to individually sort or separate subpopulations of cells.
Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light.
One of the fundamentals of flow cytometry is the ability to measure the properties of individual particles. When a sample enters a flow cytometer, the particles are randomly distributed in the 3-D space of the sample line, the diameter of which is significantly larger than the diameter of most cells.
The basic principle of flow cytometry is the passage of cells in single file in front of a laser so they can be detected, counted and sorted. Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths.
With flow cytometry, we detect and measure the physical, chemical and fluorescent characteristics of cells (or particles), o ne at a time, by passing them through laser beams and collecting the light that is emitted or scattered.
Flow Cytometry involves the use of a beam of laser light projected through a liquid stream that contains cells, or other particles, which when struck by the focused light give out signals which are picked up by detectors.
