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Prepare stain in fume hood. Avoid contact and inhalation of dyes. Acetic acid: Irritating to respiratory system. Target organ effects on respiratory system by inhalation. Corrosive. PROCEDURE: 1. Hydrate slides to distilled water.
Microwave Staining Protocol 1. Deparaffinize and hydrate to deionized water. 2. Place sections in a plastic coplin jar containing Alcian Blue pH 2.5 Stain Solution. 3. Microwave for 25 seconds at 80% power to achieve 60° C. 4. Incubate sections an additional 1 minute in heated Alcian Blue Solution. 5. Rinse sections in several changes of ...
In most protocols, sections are stained with a standard alcian blue (pH 2.5) method followed by PAS. The alcian blue stains sialomucins, sulfomucins, and proteoglycans blue. Neutral mucins are stained deep red/magenta with the PAS.
PRINCIPLE AND RESULTS: This stain is intended for use by laboratory professionals to stain routinely prepared paraffin embedded tissue specimens (in vitro) to identify acid mucins, carboxylated and sulfated mucosubstances. Strongly sulfated mucosubstances are not demonstrated. Acid mucins, carboxylated and sulfated mucosubstances are stained blue.
The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
Fixation is an essential preliminary step in IF staining in order to prevent autolysis, mitigate putrefaction, and preserve morphology while maintaining antigenicity. The ideal fixation method serves to immobilize target antigens without disturbing cellular architecture to allow antibodies maximum access to any targeted cellular components.
Sample Type Required: Deparaffinized and re-hydrated tissue sections on positively charged slides. This rinse will protect the subsequent Alcian Blue Solution from pH changes due to the introduction of water. Rinse well enough to remove excess Alcian Blue. This will help to prevent nonspecific staining. Expected Results.
