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Introduction. This lab will determine the presence or absence of amplified DNA in your samples by visualization on an agarose gel. Arthropod and Wolbachia DNA, if present, will be distinguishable based on the size, or base pair (bp) length, of the DNA molecule.
Agarose gel electrophoresis is method for separation (by size), quantifying, purification of nucleic acids fragments mixture, and analysis of DNA restriction fragments. It is one of the most widely-used techniques in biochemistry and molecular biology.
In this lab, we saw how gel electrophoresis could be used to determine the molecular composition and the specific charge. We were able to use gel electrophoresis successfully to determine how many different DNA, RNA, or proteins are present in a sample and how large they are in comparison to one another.
Agarose gel electrophoresis is a powerful separation method frequently used to analyze DNA fragments generated by restriction enzymes, and it is a convenient analytical method for separating DNA fragments of varying sizes ranging from 100 bp to 25 kb.
In general, gel electrophoresis is a process by which the macromolecules within a sample are separated from one another on the basis of size. The process can be applied to different types of macromolecules such as proteins and nucleic acid (DNA and RNA).
Develop important skills in gel electrophoresis, a widely used technique for the preparation and analysis of DNA and proteins. Prerequisite Skills: • Prior practice with micropipettors. • Familiarity with the basic principles of agarose gel electrophoresis. TEACHING TIME: 90 minutes or two class periods of 45min each materials assign ...
The objective of this experiment is to develop a basic understanding of electrophoretic theory, and gain “hands-on” familiarity with the proce-dures involved in agarose gel electrophoresis to separate biological molecules. All components are intended for educational research only.
