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Official cattle and swine slaughter establishments are required to test carcasses for generic E. coli as a means of verifying process control. This guidance document outlines sampling and microbial testing procedures that would meet this requirement.
INTRODUCTION. Under the Pathogen Reduction/HACCP Regulation, slaughter establishments are required to test carcasses for generic E. coli as a means of verifying process control. This document outlines sampling and microbial testing procedures that would meet this requirement.
Explain why generic E. coli sampling and analysis is performed in livestock (other than swine) slaughter operations. Explain why microbiological sampling and analysis is performed in swine and poultry slaughter (other than ratite) operations.
Why is this testing done? Generic, or Biotype I, Escherichia coli is found in the feces and intestinal tract of all meat and poultry animals. Finding this bacterium on a slaughtered and dressed carcass is viewed as an indication that fecal contamination has occurred.
This briefing note looks at the relevance of generic E. coli to food safety referring to fresh produce and is also relevant to all food types. With sections including transmission, testing and prevention it concludes: A positive for E. coli O157 alone may not cause illness.
Generic E. coli can be enumerated by MPN but enumeration is seldom done for pathogenic E. coli strains. Also, no pathogenic E. coli enumeration methods have been validated.
FDA Food Safety Modernization Act (FSMA) introduces generic E. coli test requirements for agricultural water
