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HIV-1 Protease Activity Assay Kit (Fluorometric) (ab211105) provides a convenient and simple method for detecting HIV-1 Protease activity in mammalian tissue samples and from purified proteins.
HIV-1 Protease Inhibitor Screening Kit (Fluorometric) (ab211106) provides a rapid, simple test suitable for high-throughput screening of HIV-1 Protease inhibitors. The assay is based on the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release a free fluorophore, which can be easily quantified using a
Standard (reverse transcriptase and protease) genotypic drug-resistance testing should be used to guide selection of an ARV regimen; if transmitted integrase strand transfer inhibitor (INSTI) resistance is a concern or for people who acquired HIV after taking long-acting cabotegravir as pre-exposure prophylaxis, testing also should include the i...
1 Μαΐ 2012 · In this chapter, the authors present basic properties of protease enzymes, sensitive fluorescent assay formats compatible with HTS, both in homogeneous and separation-based approaches. Sections on hit selection and data analysis concepts for identifying inhibitors are also included with an extensive set of literature references.
Two surrogate markers are used to monitor people with HIV: plasma HIV RNA (viral load) to assess level of HIV viremia and CD4+ T lymphocyte cell count (or CD4 count) to assess immune function. Standard (reverse transcriptase and protease) genotypic drug-resistance testing should be used to guide selection of an
5 Ιουλ 2024 · Diagnostic testing is the crucial first step in the HIV care continuum (Figure 1). [1] . Establishing a diagnosis of HIV has important implications for both HIV treatment and prevention.
21 Ιαν 2019 · Herein we report an optimal expression and solubilisation procedure to recover active HIV-1 C-SA protease enzyme from inclusion bodies. The HIV protease was expressed in seven different vectors (pET11b, pET15b, pET28a pET32a, pET39b, pET41b and pGEX 6P-1).
