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3 Ιουλ 2023 · It is crucial to follow the specific instructions that come with each kit. Basic Protocol 1 describes the extraction of DNA from human blood, tissue, or cultured cells using a column-based kit such as the QIAamp DNA Blood Mini Kit or the DNeasy Blood and Tissue Kit.
The Wizard® HMW DNA Extraction Kit is designed for isolating high-molecular-weight DNA from white blood cells, tissue culture cells, plant tissue, and Gram-positive and Gram-negative bacteria. DNA purified with this system is particularly suitable for long-read sequencing platforms when the protocol is closely followed and DNA handled carefully.
This protocol describes the extraction of HMW DNA from human whole blood. It is recommended for HiFi sequencing. Input requirements. Amount: 200 μL of human whole blood. • Yield for human whole blood will vary from 3–10 μg depending on donor WBC concentration. • Blood samples need to be ≥ 4 X 106 WBC cells/L to give ≥ 3 g HMW DNA yield.
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