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Where calcium precipitation is a problem, replacing calcium chloride with calcium borogluconate as the Ca ++ source may be helpful in obtaining reproducible plaque formation. Douglas et al . (1974) reported that plaque size could be increased by the addition of magnesium sulphate to assay media.
20 Νοε 2022 · This paper claims: Addition of divalent ions (CaCl 2 and MgCl 2) may also facilitate plaque formation through aiding phage adsorption to the bacterial receptor. But how does CaCl 2 aid phage adsorption exactly? And by extension, is there an ideal concentration of CaCl 2 that maximizes the adsorption when growing bacteria in liquid media?
24 Απρ 2009 · To achieve 100% correlation between plaque and colony counts, the optimized phage assay includes the following: (i) resuspension of the samples to be tested in Middlebrook 7H9 broth containing 10% oleic acid-albumin-dextrose-catalase and 2 mM calcium chloride, followed by overnight incubation at 37°C before performance of the phage assay; (ii ...
19 Ιουν 2024 · Discover the role of calcium chloride in food production, its safety profile, various applications, and regulatory guidelines. Team Chemicals, your trusted source for calcium chloride, explains.
18 Μαΐ 2015 · Defining the requirement for calcium in lactococcal phage infection. Plaque assays involving a range of calcium chloride concentrations from 0 to 10 mM, or the presence of the chelating agent EDTA were performed. Out of the 8 phages tested, only 1706 required calcium for plaque formation (Table 2).
Since concentration-related side effects, the desired plaque reduction can be accomplished by 10 ml 0.1% of chlorhexidine solution for one or two daily applications. Reduce chlorhexidine levels even further in mentally ill children to help reduce discolorations.
Arterial vascular calcification (VC) represents formation of calcium phosphate deposits on the interior of arteries, which could restrict blood flow leading to heart health problems, including morbidity and mortality.
