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  1. 12 Σεπ 2023 · A sequential co-culture procedure using the three cell types of a MU, MN, myoblast, and Schwann cell was designed to construct a co-differentiating tri-culture on a bilayer hydrogel matrix. We utilized a µ-molded hydrogel with an additional Matrigel layer to form the bilayer hydrogel device.

  2. 22 Ιαν 2021 · We report a method to generate a 3D motor neuron model with segregated and directed axonal outgrowth. iPSC-derived motor neurons are cultured in extracellular matrix gel in a microfluidic...

  3. 1 Ιαν 2009 · Primary neuronal and Schwann cell cultures. The blue panel contains images of embryonic DRG neurons, the yellow panel contains images of embryonic motor neurons, the green panel shows adult DRG neurons, and the gray panel contains Schwann cells.

  4. 19 Μαρ 2021 · In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as a possible downstream application.

  5. 12 Οκτ 2015 · Here, we introduce a simple and reproducible myelination culture system using mouse motor neurons (MNs) and Schwann cells (SCs). Dissociated motor neurons are plated on a feeder layer of...

  6. 30 Μαρ 2021 · NSC-34 is a hybrid cell line generated by the fusion of mouse embryonic motor neuron-enriched spinal cord cells with mouse neuroblastoma N18TG2 . This cell line is a relevant model for the study of motor neuron biology and expresses many motor neuron-like properties (for overview, see [14,15,16]).

  7. Neuronal cell culture refers to the technique of growing nerve cells in a controlled environment outside of the body, which is essential for studying the effects of chemotherapeutic drugs on the nervous system and developing protective strategies. AI generated definition based on: Experimental Neurology, 2020

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