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  1. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Blast

      Blast - Basic Local Alignment Search Tool - BLAST

    • Protein Blast

      Protein Blast - Basic Local Alignment Search Tool - BLAST

    • Blastx

      Blastx - Basic Local Alignment Search Tool - BLAST

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

    • Blast FAQ

      The NCBI cannot provide compute resources for large-scale...

    • Recent Results

      Enter a BLAST RID in the RID box below and click Go to find...

    • Blast Download Page

      Magic-BLAST ¶ Magic-BLAST is a tool for mapping large...

    • Cobalt

      COBALT is a multiple sequence alignment tool that finds a...

  2. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.

  3. COBALT is a multiple sequence alignment tool that finds a collection of pairwise constraints derived from conserved domain database, protein motif database, and sequence similarity, using RPS-BLAST, BLASTP, and PHI-BLAST.

  4. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.

  5. 1 Ιουλ 2007 · FASTA finds short common patterns in query and database sequences and joins these into an alignment. BLAST is similar to FASTA, but gains a further increase in speed by searching only for rarer, more significant patterns in nucleic acid and protein sequences.

  6. A global algorithm returns one alignment clearly showing the difference, a local algorithm returns two alignments, and it is difficult to see the change between the sequences. The global alignment at this page uses the Needleman-Wunsch algorithm.

  7. This tool is based on NCBI BLAST and uses the latest implementation of the BLAST algorithm and a special sequence database known as EMVec.

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