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  1. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Blast

      Blast - Basic Local Alignment Search Tool - BLAST

    • Protein Blast

      Protein Blast - Basic Local Alignment Search Tool - BLAST

    • Blastx

      Blastx - Basic Local Alignment Search Tool - BLAST

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

  2. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.

  3. www.ncbi.nlm.nih.gov › guide › howtoDownload NCBI Software

    How to: Download NCBI Software. Starting with the software program of interest... BLAST (aligns nucleotide and protein sequences) NCBI ToolBox (C/C++ source code for NCBI software) CD-Tree (views and edits protein alignments in CD records) Cn3D (3D structure viewer and alignment editor) e-PCR (finds sequence tagged sites in DNA sequences)

  4. www.ncbi.nlm.nih.gov › comparative-genomics-resource › analysis-toolsAnalysis tools - NCBI - NLM

    Use the NCBI Toolkit to compare genomic sequences BLAST. Find regions of similarity between biological sequences with the Basic Local Alignment Search Tool (BLAST). There are many ways to BLAST: SmartBLAST: find proteins highly similar to your query ; Primer-BLAST: design primers specific to your PCR template

  5. A BLAST search has four components: query, database, pr ogram, and search purpose/goal. To discuss effective BLAST program selection, we first need to know what databases are available and what sequences these databases contain.

  6. BLAST is a computer algorithm that is available for use online at the National Center for Biotechnology Information (NCBI) website, as well as many other sites. BLAST can rapidly align and...

  7. Using “Align Two Sequences”, two groups of sequences can be directly compared. Search results are identified using the assigned unique request ids (RIDs) and are accessible for up to 36 hours. With an NCBI login [2], a search strategy can be saved for future reference.

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