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  1. Align two or more sequences Help Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences.

    • Protein Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Tblastn

      PSI-BLAST allows the user to build a PSSM (position-specific...

  2. This document first introduces the BLAST databases available from NCBI (in Section 2). The actual guide (Section 3) divides BLAST searches into several categories according to the nature and size of the input query and the primary goal of the search.

  3. Pairwise alignments and multiple alignments are the basic tools to compare sequences. BLAST is the most frequently used bioinformatics program to compare your own sequence (query sequence) to all sequences in a database (subject sequences) based on local pair-wise alignments. The outcome of the BLAST analysis provides qualitative information

  4. A global algorithm returns one alignment clearly showing the difference, a local algorithm returns two alignments, and it is difficult to see the change between the sequences. The global alignment at this page uses the Needleman-Wunsch algorithm.

  5. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  6. In a BLAST search form, the “Blast 2 sequences” checkbox (A) activates the “Align Two Sequences” function and dis-plays the subject sequence input box (B) while removing the elements pertaining to database selection. The “Align Two Sequences” also adds a new set of parameters for fine tuning searches:

  7. 28 Φεβ 2011 · Introduction: The Basic Local Alignment Search Tool (BLAST) is a program that can detect sequence similarity between a Query sequence and sequences within a database. The ability to detect sequence homology allows us to identify putative genes in a novel sequence.

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