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  1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

    Primer-BLAST uses Primer3 and BLAST to design primers for PCR based on a template sequence. You can enter your own primers, set parameters for primer specificity, melting temperature, exon/intron selection, and search mode.

    • Publication

      Primer-BLAST was developed at NCBI to help users make...

    • Blast

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • PMC Free Article

      Primer-BLAST is a general purpose target-specific PCR primer...

  2. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity - National...

    Primer BLAST performs only a specificity check when a target template and both primers are provided. In the Primer Pair Specificity Checking Parameters section, select the appropriate source Organism and the smallest Database that is likely to contain the target sequence.

  3. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-Blast results - National Center for Biotechnology...

    Primer-BLAST Results Help. Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse ...

  4. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

    Primer-BLAST helps users make primers that are specific to intended PCR target by using Primer3 and BLAST algorithms. It screens primers against user-selected database to avoid non-specific amplifications and provides citation and details.

  5. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

    Allow primers to amplify mRNA splice variants. This will make it much easier to find gene-specific primers if you do not need to distinguish between splice variants. Specify an organism for database search if you are only amplifying DNA from a specific organism.

  6. blast.ncbi.nlm.nih.govBLAST: Basic Local Alignment Search Tool

    The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  7. www.ncbi.nlm.nih.gov › pmc › articlesPrimer-BLAST: A tool to design target-specific primers for...

    18 Ιουν 2012 · Primer-BLAST is a general purpose target-specific PCR primer design tool that offers a level of sensitivity and usability not found in other tools.

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