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  1. Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.

    • Protein Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Tblastn

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • NCBI Blast

      We would like to show you a description here but the site...

  2. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  3. 6 Αυγ 2024 · The Basic Local Alignment Search Tool (BLAST) finds regions of similarity between sequences. The program compares nucleotide or protein sequences and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

  4. The NCBI Multiple Sequence Alignment Viewer (MSA) is a graphical display for nucleotide and protein sequence alignments. Review documentation or watch a video tutorial. To see your own alignment, your data. Examples of various alignment styles: Protein alignment with no anchor set. Protein alignment, anchor set to ACI28628.

  5. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.

  6. In a BLAST search form, the “Blast 2 sequences” checkbox (A) activates the “Align Two Sequences” function and dis-plays the subject sequence input box (B) while removing the elements pertaining to database selection. The “Align Two Sequences” also adds a new set of parameters for fine tuning searches:

  7. Select “Nucleotide BLAST” under Web BLAST. Check the box “Align two or more sequences” to load a second query box “Enter Subject Sequence.”. To identify variation in different sequences, those sequences must be compared to a standard sequence called a reference sequence.

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