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13 Μαΐ 2024 · The principle of Nylander’s Test revolves around the inherent chemical properties of certain carbohydrates, specifically those possessing an aldehyde group or a free ketone. In the realm of biochemistry, these functional groups play a pivotal role in the reaction mechanism of the test.
This manual includes detailed view of the following: Laboratory Safety Precautions, Chemical Reagent Preparation, Buffer and Chemical Solution Preparation, Staining Solution Preparation, Indicators and Dye Solution Preparation, Preparation of Special Reagents, Known and Suspected List of Carcinogens and References.
Nylander's test is a chemical test used for detecting the presence of reducing sugars. Glucose or fructose reduces bismuth oxynitrate to bismuth under alkaline conditions. When Nylander's reagent, which consists of bismuth nitrate, potassium sodium tartrate and potassium hydroxide, is added to a solution with reducing sugars, a black ...
24 Οκτ 2017 · N-Bromosuccinimide (NBS) is a chemical reagent used for radical substitution and electrophilic addition reactions. It can be conveniently prepared by adding bromine to an ice-cooled solution of succinimide in alkali.
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28 Φεβ 2020 · Take 5 ml sample solution in a test tube, add 5–8 drops of Nylander’s reagent, boil for 3 min on water bath, and cool. Observation. Black precipitates develop after a few minutes (bismuth subnitrate reduced to black bismuth).
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