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We will start with an introduction to microscopy, its history and simultaneously introduce concepts of optics that are important to understand how microscopes work. This free course introduces concepts in detectors, fluorescence microscopy, sample preparation and confocal microscopy.
Phase-contrast microscopy. Phase contrast is an optical contrast technique for making unstained transparent objects visible under the optical microscope. An annulus aperture is placed in the front focal plane of the condenser and limits the angle of the penetrating light waves.
Optical microscopy is an imaging methodology with a diffraction-limit given by the wavelength(s) of light used to generate a diffraction pattern and the aperture of the objective lens used to collect the diffracted light source.
Optical super-resolution microscopic approaches, including single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, and structured illumination microscopy (SIM) have been invented, beating the Abbe diffraction limit presented on light microscopes.
Applications of Optical Microscopy 1. Crystal morphology and symmetry • Crystal fragments (characteristic shape) • Classify isotropic and anisotropic substances • Check possible symmetry (parallel extinction) 2. Phase identification, purity and homogeneity • Standard optical data (refractive indices and optical axes) for comparison
28 Οκτ 2023 · Compared to other techniques in life science, optical microscopy makes it possible for us to visualize biology in its physiological context. In a short span of less than three decades,...
24 Ιαν 2019 · The SRM family encompasses a range of far-field optical techniques that exploit a number of chemical and physical principles to overcome the diffraction limit and enable nanometric-resolved...
