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26 Νοε 2020 · Be able to set up and use a microscope to study a range of specimens. Understand the steps required to prepare metallographic, ceramic and polymer specimens. This Teaching and Learning Package (TLP) provides an introduction to the use of optical microscopes.
- 10.5: Polarised Light
The retardation also depends on the orientation of the...
- 10.1: Introduction
Reflected-light microscopy is used for a range of materials,...
- 10.7: Summary
See techniques for optical microscopy for more information...
- Ceramics and Polymers
To prepare ceramic specimens for transmitted light...
- 10.2: Sample Preparation
Polishing. Polishing discs are covered with soft cloth...
- 10.4: Microscope
When looking down the eyepiece and using coarse focus, you...
- 10.8: Questions
This page titled 10.8: Questions is shared under a CC...
- Resolution and Imaging
Numerical Aperture; Airy Discs; The limit of resolution (or...
- 10.5: Polarised Light
9 Ιαν 2017 · Optical Microscopy uses visible light and a system of lenses to magnify images of small samples. Optical microscopy is used extensively in microelectronics, nanophysics, biotechnology...
Phase-contrast microscopy. Phase contrast is an optical contrast technique for making unstained transparent objects visible under the optical microscope. An annulus aperture is placed in the front focal plane of the condenser and limits the angle of the penetrating light waves.
In this article, we will discuss some of the most important and useful specimen preparation techniques in modern optical microscopy. First, we will cover the methods used for immobilizing and selectively staining cells or parts of cells.
Applications of Optical Microscopy 1. Crystal morphology and symmetry • Crystal fragments (characteristic shape) • Classify isotropic and anisotropic substances • Check possible symmetry (parallel extinction) 2. Phase identification, purity and homogeneity • Standard optical data (refractive indices and optical axes) for comparison
Optical microscopy is an imaging methodology with a diffraction-limit given by the wavelength(s) of light used to generate a diffraction pattern and the aperture of the objective lens used to collect the diffracted light source.
1 Ιαν 2010 · This chapter covers, in a very brief and introductory way, some of the fundamental aspects of modern optical microscopy, such as contrast techniques, spatial resolution topics, and high-speed acquisition solutions.
