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Primer-BLAST uses Primer3 and BLAST to design primers for PCR based on a template sequence. You can enter your own primers, set parameters, and check primer specificity and melting temperatures.
- Primer-BLAST
Primer-blast tries to find target-specific primers by...
- Tips for Finding Specific Primers
The reason is that an accession or GI carries accurate...
- PubMed
Primer-BLAST allows users to design new target-specific...
- Blast
The Basic Local Alignment Search Tool (BLAST) finds regions...
- PMC Free Article
Primer-BLAST allows users to design new target-specific...
- Primer-BLAST Help
Primer-BLAST Results Help. Primer-BLAST was developed at...
- Primer design for RT-PCR
Primer design is a crucial initial step in any experiment...
- Primer-BLAST
18 Ιουν 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
18 Ιουν 2012 · Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on exon/intron locations and excluding single nucleotide polymorphism (SNP) sites in primers.
Primer-BLAST Results Help. Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses BLAST and global alignment algorithm to screen primers against user-selected database in order to avoid primer pairs (all combinations including forward-reverse ...
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity.
Primer BLAST performs only a specificity check when a target template and both primers are provided. In the Primer Pair Specificity Checking Parameters section, select the appropriate source Organism and the smallest Database that is likely to contain the target sequence.
