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To 1-3 µg RNA, add 0.5-3X volumes Formaldehyde Load Dye. To simply check the RNA on a denaturing gel, as little as 0.5X Formaldehyde Load Dye can be used, but to completely denaturate the RNA, e.g. for Northern blots, use 3X volumes of Formaldehyde Load Dye.
RNA fragments are analyzed on The FlashGel TM System using the same fast and simple procedure used for DNA. Separation is complete in <8 minutes and RNA samples are ready for imaging within 10-20 minutes. The FlashGel TM System for RNA is 5-20X more sensitive than ethidium bromide gels.
Our Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb. The 8-well gels are 1.25% RNase-free agarose (A9539) prepared using 1X MOPS buffer with no denaturants.
gels (formaldehyde-agarose or polyacrylamide-urea) reduce effects of RNA secondary structure and provide more accurate size determinations than native gels, but are difficult to run and stain. Denaturing gels are
Formaldehyde serves primarily as a denaturing agent for RNA during agarose gel electophoresis. An additional useful property of formaldehyde is its inhibitory effect on RNases [ 5 ], which helps maintain RNA integrity during separation and gel handling.
Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5–10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
Purchase a 36.5-38% formaldehyde solution suitable for fixing cells and tissue sections, as well as agarose gel electrophoresis, for research studies at Sigma-Aldrich.
