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We describe a method to facilitate electrophoretic separation of high molecular weight RNA species, such as ribosomal RNAs and their precursors, on agarose-formaldehyde gels.
- Denaturation and electrophoresis of RNA with formaldehyde
Electrophoretic size fractionation can be used to denature...
- Denaturation and electrophoresis of RNA with formaldehyde
2 Φεβ 2015 · Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5-10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
1 Οκτ 2013 · We describe a method to facilitate electrophoretic separation of high-molecular-weight RNA species, such as ribosomal RNAs and their precursors, on agarose–formaldehyde gels. Two alternative “p K -matched” buffer systems were substituted for the traditionally used Mops-based conductive medium.
25 Φεβ 2019 · Chromatin isolation by RNA purification (ChIRP) 30,39 and capture hybridization analysis of RNA targets (CHART) 31, in contrast, use formaldehyde to cross-link RNA to proteins.
Electrophoretic size fractionation can be used to denature and separate large mRNA molecules (0.5–10 kb) on formaldehyde-containing agarose gels. Formaldehyde contains a carbonyl group that reacts to form Schiff bases with the imino or amino groups of guanine, adenine, and cytosine.
16 Ιαν 2023 · Recent advances in high throughput sequencing enabled the development of methods for probing RNA structures on a transcriptome-wide scale - termed the RNA structurome. Here we review the state-of-the-art technologies for probing the RNA structurome, and highlight insights gained from these studies.
For procedures such as Northern analysis, in which RNA is transferred or blotted from the gel to a solid matrix for subsequent hybridization (see Chapters 15 and 17), the optimal balance between electrophoretic resolution and efficiency of transfer is achieved with a 1–1-2% agarose gel (see refs. 1 – 3).
