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10 Αυγ 2022 · Positive (+ve) Ornithine Decarboxylase. Negative (-ve) Biochemical Test and Identification of Staphylococcus aureus. It is Gram positive, Coagulase positive, catalase and oxidase positive non-motile bacteria.
There are only a few species of Staphylococcus that are positive for the coagulase test (see table below), and S. aureus is the most common. Since there are 2 kinds of coagulase enzyme—bound and free---there are 2 different tests that can be used to identify these enzymes.
17 Ιουλ 2023 · These organisms can grow aerobically or anaerobically (facultative) and at temperatures between 18 C and 40 C. Typical biochemical identification tests include catalase positive (all pathogenic Staphylococcus species), coagulase positive (to distinguish Staphylococcus aureus from other Staphylococcus species), novobiocin sensitive (to ...
Ideally a Gram stain of the colony should be performed and tests made for catalase and coagulase production, allowing the coagulase-positive S aureus to be identified quickly. Another very useful test for S aureus is the production of thermostable deoxyribonuclease.
S. aureus also produces catalase; when applied to colony material, the catalase test is a rapid, useful test to distinguish staphylococci from other Gram-positive bacteria such as streptococci. S. aureus is a facultative anaerobe that grows by aerobic respiration or by fermentation, which yields principally lactic acid.
18 Ιαν 2022 · The coagulase test is an important test that differentiates the species of the genus Staphylococci into two groups; Coagulase positive Staphylococci and Coagulase Negative Staphylococci. The coagulase enzyme acts as a virulence factor in some organisms as it interacts with the fibrinogen present on the host’s cell surface.
Other infections require samples of blood or infected fluids, which are sent to a laboratory to grow (culture), identify, and test the bacteria. Laboratory results confirm the diagnosis and determine which antibiotics can kill the staphylococci (called susceptibility testing).
