Αποτελέσματα Αναζήτησης
16 Φεβ 2021 · Here authors use atomic force microscopy and atomistic molecular dynamics simulations, to resolve structures of negatively-supercoiled DNA minicircles at base-pair resolution.
- Metrics
We would like to show you a description here but the site...
- Full Size Image
We would like to show you a description here but the site...
- Metrics
12 Οκτ 2015 · By electron cryo-tomography, biochemical assays and molecular dynamics simulations, here the authors show that supercoiled DNA minicircles adopt unique and wide distributions of...
28 Σεπ 2021 · DNA in cells is supercoiled and constrained into loops and this supercoiling and looping influence every aspect of DNA activity. We show here that negative supercoiling transmits mechanical...
24 Νοε 2023 · By fitting a simple kinetic model to the fluorescence intensity traces (Figure 5A, B and E, F) at the transition between supercoiled and nicked DNA, we are able to determine the on- and off-rate of SYTOX Orange.
DNA supercoiling defines the phenomenon whereby the actual linking number Lk differs from Lk 0. The handedness of supercoils is positive when they are in the same direction as the double helix (right‐handed), that is, DNA is over‐wound (Lk >Lk 0), and negative when DNA is under‐wound (Lk <Lk 0).
Supercoiling is a fundamental property of DNA and chromatin. It is modulated by polymerase and topoisomerase activities and, through regulated constraint, by DNA/chromatin binding proteins. As a non-covalent and elusive topological modification, ...
20 Αυγ 2010 · Supercoiling affects transcription, DNA replication, and chromosomal segregation. Consequently the cell must fine-tune supercoiling to optimize these key processes. Here, we summarize how supercoiling is generated and review experimental and theoretical insights into supercoil relaxation.
