Αποτελέσματα Αναζήτησης
28 Σεπ 2021 · Our results provide a model for how DNA can form short loops, which are required for many essential processes, and how cells may use DNA loops to position nicks to facilitate repair.
The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis. The 5 kb plasmid has an increased intensity to serve as a reference band.
16 Φεβ 2021 · Here authors use atomic force microscopy and atomistic molecular dynamics simulations, to resolve structures of negatively-supercoiled DNA minicircles at base-pair resolution.
24 Νοε 2023 · Our model has broad implications for the detection and quantification of DNA, including the use of psoralen for UV-induced DNA crosslinking to quantify torsional tension in vivo, and for the modulation of DNA binding in cellular contexts.
20 Αυγ 2010 · Supercoiling affects transcription, DNA replication, and chromosomal segregation. Consequently the cell must fine-tune supercoiling to optimize these key processes. Here, we summarize how supercoiling is generated and review experimental and theoretical insights into supercoil relaxation.
19 Απρ 2018 · Supercoiling can induce competition among the energetically favored secondary structures, which are analyzed in the DZCBtrans model; this model provides a comprehensive prediction of the secondary structure in a given DNA molecule at equilibrium as a function of σ, temperature, and ionic strength.
24 Απρ 2017 · Key approaches to study DNA supercoiling in eukaryotes are (1) centrifugation-based or electrophoresis-based techniques in which supercoiled plasmids extracted from eukaryotic cells form a compacted writhed structure that migrates at a rate proportional to the level of DNA supercoiling; (2) in vivo approaches based on the preferential ...
