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However, guidelines for general testing of cell lines currently do not exist—especially for lines that are not obtained from a cell bank or are derived locally. In this technical document, ATCC recommends basic benchmark verification tests that can be employed by any lab and included in publication.
Test your cell lines: When you receive a cell line into the laboratory. After 10 passages. After preparing a cell bank. When in doubt. Learn about cell authentication and ATCC STR analysis in two minutes. ATCC human cell STR profiling in three easy steps.
Introduction: Misidentification of cell lines Cell line authentication. Short tandem repeat (STR) profiling technique. Mouse STR profiling. When and why should you authenticate Summary. Consequences of using misidentified cell lines. Loss of cell line. Loss of time and money. Misinformation in the public domain. Discordant or irreproducible results
8 Νοε 2012 · Short tandem repeat (STR) profiling is the recommended approach for authentication testing of human cell lines. To improve its effectiveness, however, a method by which STR can account for genetic drift arising from the passage of malignant cells is needed.
2 Μαρ 2023 · cell line (non-cancer). For the authentication of cell lines, at least 13 nuclear STR loci are required plus amelogenin to determine the sex (XX or XY). Unfortunately, male-derived cells often lose the Y chromosome in culture [6], so sex determination can only be considered indicative.
Simple observation, monitoring, and documentation of cell morphology and behavior, including growth rates, provide early warning and should be standard practice. Changes may indicate microbial contamination, genotypic drift due to high passage number, or cross-contamination with another cell line.
9 Ιαν 2014 · Guide to Human Cell Line Authentication . This protocol sets out the essential steps to follow when authenticating human cell lines. SUMMARY. Send cells or DNA to an experienced supplier of STR profile testing, working according to the human cell line authentication Standard published by ANSI (1).
