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28 Φεβ 2020 · The green color can be quantitated using UV spectrophotometer or colorimeter at 665 nm. Download protocol PDF. Principle. Pentose sugar present in ribonucleic acid reacts with hot acid to form furfural, which on reaction with orcinol and ferric chloride produces green color.
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- Table 1
18 Απρ 2022 · Bial’s test is a chemical test performed to detect the presence of pentoses and pentosans (derivatives of pentoses). A derivation of this test termed the Bial’s Orchintest is performed to detect the presence of RNA in solutions.
11 Απρ 2019 · So, the basic objective of this test is to estimate RNA by orcinol method. Principle: The method usually involves the pentose conversion and ribose in the presence of hot acid to furfural that again reacts with orcinol to form a green colour.
7 Απρ 2021 · What Is Bial’s Test? Bial’s test named after a German physician Manfred Bial, is a chemical test for detection of presence of pentoses. Bial’s test reagent contains concentrated Hydrochloric acid (HCl) as a dehydrating acid, orcinol and traces of ferric chloride as condensation reagent.
In the orcinol assay (b), the positive controls are ribose (0.15 mg/ml), RNA (7.5 mg/ml), and DNA (0.45 mg/ml), while water and BSA protein (0.45 mg/ml) are negative controls. In the diphenylamine assay (c), calf thymus DNA (0.45 mg/ml) is shown as a positive control, and water, yeast extract RNA (7.5 mg/ml), ribose (0.15 mg/ml), and BSA (0.45 ...
Pipette out standard RNA solution in arrange of 0–2 mL into a series of test tube and make up the volume of each tube 2 mL with distilled water. Add 3 mL of orcinol reagent to each tube for standard solution. For test solution : Take 2 mL of nucleic acid sodium (isolated from tissue source.)
Aim: To estimate the concentration of RNA by orcinol reaction. Principle: This is a general reaction for pentoses and depends on the formation of furfural when the pentose is heated with concentrated hydrochloric acid.
