Αποτελέσματα Αναζήτησης
18 Απρ 2018 · Procedure of the Total Platelet Count by Microdilution Method. ⇒ Fill the WBC pipette up to the 0.5 mark with the blood specimen and wipe out the pipette externally to avoid false high results. ⇒ Fill the same pipette with the Platelet diluting fluid (i.e. the Rees – Ecker Fluid) up to the mark 11.
Hema 2 courtesy of Prof Caravana
The normal values for the platelet count done by an experienced technician using the Rees and Ecker method were: mean, 409,000 per cu. mm.; standard deviation, 68,000 per cu. mm.; normal range (M ± 2.σ), 273,000 per cu. mm. to 545,000 per cu. mm.
Direct (Rees-Ecker Method) This method used the erythrocyte diluting pipet; whole blood is diluted with a solution containing brilliant cresyl blue, which stains the platelets a light bluish color. The platelets are then counted using a standard hemocytometer and bright field microscopy.
The essentials of a good method for platelet counting are: (1) an efficient anticoagulant; (2) absence of contact between the undiluted blood and any instrument; (3) preservation and fixation of red blood corpuscles; (4) low specific gravity of diluting solution, and (5) a dye that will readily stain the platelets.
Type of direct platelet count PROCEDURE: 1. The Unopette is to mix blood and diluting fluid. Mix diluted blood samples for 10-15 minutes; Using a phase hemocytometer charge the mixed dilution; The “P” in the phase hemocytometer suggests where to count platelets; PHASE CONTRAST MICROSCOPE
The essentials of a good method for platelet counting are: (1) an efficient anticoagulant; (2) absence of contact between the undiluted blood and any instrument; (3) preservation and fixation of red blood corpuscles; (4) low specific gravity of diluting solution, and (5) a dye that will readily stain the platelets.
